TRIP13 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIP13 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (4)

Cat no : 19602-1-AP

Synonyms

16E1 BP, 16E1BP, PCH2, TR interacting protein 13, TRIP 13, TRIP13

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Validation Data Gallery

• 

  WB analysis using 19602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19602-1-AP (TRIP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19602-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19602-1-AP (TRIP13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19602-1-AP

  IP result of anti-TRIP13 (IP:19602-1-AP, 3ug; Detection:19602-1-AP 1:400) with mouse testis tissue lysate 6000ug.

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Tested Applications

• Positive WB detected in: HT-1080 cells, mouse testis tissue, Jurkat cells
• Positive IP detected in: mouse testis tissue

It works great for western. By Dr. Soory

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 19602-1-AP is TRIP13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIP13 fusion protein Ag6595
• Full Name: thyroid hormone receptor interactor 13
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC000404
• Gene Symbol: TRIP13
• Gene ID (NCBI): 9319
• RRID: AB_10642702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIP13 gene encodes thyroid receptor-interacting protein 13, that interact with thyroid hormone receptors, also known as human papillomavirus type 16 E1 protein-binding protein (16E1BP). This protein belongs to the AAA ATPase family and the PCH2 subfamily. TRIP13 gene may be one of the several playing roles in early-stage non-small cell lung cancer. TRIP13 also functions in chromosome recombination and chromosome structure development during meiosis, and it is required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation.

Publications

Species	Application	Title
mouse	WB	Sci Adv; SKP1 drives the prophase I to metaphase I transition during male meiosis.; Authors - Yongjuan Guan
human	WB	Nat Commun; TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer.; Authors - Rajat Banerjee ; KD Validated
	IF	Cell Prolif; ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression; Authors - Xu Zhou
human	IHC	Mol Oncol; TRIP13 promotes metastasis of colorectal cancer regardless of p53 and microsatellite instability status.; Authors - Sumit Agarwal
human	WB,IHC,IP	Cell Death Dis; TRIP13 promotes tumor growth and is associated with poor prognosis in colorectal cancer.; Authors - Nengquan Sheng
mouse	IF	PLoS Genet; The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.; Authors - Laura Gómez-H

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iru (Verified Customer) (06-25-2020)

This antibody works pretty well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Min (Verified Customer) (11-11-2019)

This is very clear and strong antibody

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Head and neck cancer cells

TRIP13 Antibody Western Blot,Immunoprecipitation, validation (1:5000 dilution) in Head and neck cancer cells (Cat no:19602-1-AP)

FH

Iru (Verified Customer) (06-21-2019)

This antibody doesn't work well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Priyanka (Verified Customer) (12-18-2018)

• Applications: Western Blot,