TRIM69 Polyclonal antibody

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  WB analysis of mouse testis using 12951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12951-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12951-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12951-1-AP (TRIM69 antibody) at dilution of 1:100 (under 10x lens).

• Featured Product
• KD/KO Validated

TRIM69 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12951-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12951-1-AP targets TRIM69 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM69 fusion protein Ag4074
• Full Name: tripartite motif-containing 69
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC033314
• Gene Symbol: TRIM69
• Gene ID (NCBI): 140691
• RRID: AB_2208974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86WT6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

Publications

Species	Application	Title
human	WB,IHC,IF,IP,CoIP	Oncogene; TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD; Authors - Linqing Sun ; KD Validated
Human,monkey	WB	Front Immunol; DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection.; Authors - Huiyuan Jing
human	WB	Cell Physiol Biochem; Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues.; Authors - Yuanyuan Chen
human	WB	J Virol; Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.; Authors - Tonya Kueck
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
mouse	IF, WB	J Biomed Res; The testis-specifically expressed gene Trim69 is not essential for fertility in mice.; Authors - Xi He ; KO Validated