TRIM59 Polyclonal antibody, PBS Only

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  WB analysis using 28575-1-AP (same clone as 28575-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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  WB analysis using 28575-1-AP (same clone as 28575-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 28575-1-AP (same clone as 28575-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28575-1-AP (same clone as 28575-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28575-1-AP (same clone as 28575-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28575-1-AP (same clone as 28575-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 28575-1-AP (same clone as 28575-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM59 antibody (28575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28575-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRIM59 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28575-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

28575-1-PBS targets TRIM59 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM59 fusion protein Ag29560
• Full Name: tripartite motif-containing 59
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_173084
• Gene Symbol: TRIM59
• Gene ID (NCBI): 286827
• RRID: AB_2918177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWR1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.