TRIM54 Polyclonal antibody

TRIM54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21074-1-AP

Synonyms

MURF, MURF 3, MuRF3, RING finger protein 30, RNF30, TRIM54, tripartite motif containing 54

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Validation Data Gallery

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  WB analysis of mouse heart using 21074-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21074-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21074-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21074-1-AP (TRIM54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 21074-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21074-1-AP (TRIM54 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 21074-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRIM54 antibody 21074-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human skeletal muscle tissue, human heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

21074-1-AP targets TRIM54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM54 fusion protein Ag15229
• Full Name: tripartite motif-containing 54
• Calculated Molecular Weight: 358 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC141807
• Gene Symbol: TRIM54
• Gene ID (NCBI): 57159
• RRID: AB_10694832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).

Publications

Species	Application	Title
human	WB	Front Oncol; Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.; Authors - Jinrong Zhu
human	WB	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu
human	WB	Hepatol Commun; Sorafenib induces ferroptosis by promoting TRIM54-mediated FSP1 ubiquitination and degradation in hepatocellular carcinoma; Authors - Man-Ru Liu