TRIM47 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM47 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 26885-1-AP

Synonyms

GOA, RING finger protein 100, RNF100, TRIM47, tripartite motif containing 47

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Validation Data Gallery

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  WB analysis of U2OS using 26885-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26885-1-AP (TRIM47 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26885-1-AP

  IP result of anti-TRIM47 (IP:26885-1-AP, 4ug; Detection:26885-1-AP 1:300) with U2OS cells lysate 2000ug.

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human kidney tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 26885-1-AP is TRIM47 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM47 fusion protein Ag22868
• Full Name: tripartite motif-containing 47
• Calculated Molecular Weight: 638 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC017299
• Gene Symbol: TRIM47
• Gene ID (NCBI): 91107
• RRID: AB_2880669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Publications

Species	Application	Title
human	WB,IHC	Neoplasma; Trim47 overexpression correlates with poor prognosis in gastric cancer.; Authors - YuJian Xia
human,mouse	WB,IHC,IP	Signal Transduct Target Ther; TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.; Authors - Yisong Qian ; KD Validated
human	WB,IHC	In Vitro Cell Dev Biol Anim; TRIM47 promotes glioma angiogenesis by suppressing Smad4; Authors - Zifan Wang ; KD Validated
human,mouse	WB,IHC,IF	Clinics (Sao Paulo); TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling; Authors - Xi Wang ; KD Validated
rat	WB	Exp Brain Res; Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats; Authors - Yingjun Zhu ; KD Validated
human	WB,IF	Comput Struct Biotechnol J; Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress; Authors - Rui Su