TRIM44 Monoclonal antibody

TRIM44 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1B12H12

Cat no : 66249-1-Ig

Synonyms

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

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Validation Data Gallery

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  WB analysis of MCF-7 using 66249-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66249-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66249-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human testis tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66249-1-Ig targets TRIM44 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM44 fusion protein Ag18771
• Full Name: tripartite motif-containing 44
• Calculated Molecular Weight: 344 aa, 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC024031
• Gene Symbol: TRIM44
• Gene ID (NCBI): 54765
• RRID: AB_2881637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.

Publications

Species	Application	Title
human	WB	J Immunol; Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.; Authors - Zhangchuan Xia
human	WB	J Immunol; NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.; Authors - Zhangchuan Xia
human	IF	Genes Genomics; Overexpression of TRIM44 mediates the NF-κB pathway to promote the progression of ovarian cancer; Authors - Yang Yu