TRIM40 Monoclonal antibody, PBS Only

TRIM40 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5D3

Cat no : 67073-1-PBS

Synonyms

TRIM, RNF35, Probable E3 NEDD8-protein ligase, EC:2.3.2.27, E3 ubiquitin ligase TRIM40

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Validation Data Gallery

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  WB analysis using 67073-1-Ig (same clone as 67073-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig small intestine using 67073-1-Ig (same clone as 67073-1-PBS)

  pig small intestine tissue were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67073-1-Ig (same clone as 67073-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67073-1-Ig (TRIM40 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 67073-1-Ig (same clone as 67073-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 67073-1-Ig (same clone as 67073-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67073-1-Ig (TRIM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67073-1-Ig (same clone as 67073-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM40 antibody (67073-1-Ig, Clone: 1C5D3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67073-1-PBS in a different storage buffer formulation.

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Product Information

67073-1-PBS targets TRIM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM40 fusion protein Ag21660
• Full Name: tripartite motif-containing 40
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 26-36 kDa
• GenBank Accession Number: BC060785
• Gene Symbol: TRIM40
• Gene ID (NCBI): 135644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.