TRIM37 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13037-1-AP

Synonyms

RING-type E3 ubiquitin transferase TRIM37, POB1, MUL, KIAA0898, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13037-1-AP (TRIM37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13037-1-AP

  IP result of anti-TRIM37 (IP:13037-1-AP, 4ug; Detection:13037-1-AP 1:800) with mouse brain tissue lysate 1800 ug.

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  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13037-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13037-1-AP (TRIM37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13037-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using TRIM37 antibody (13037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U-87 MG using 13037-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using TRIM37 antibody (13037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

13037-1-AP targets TRIM37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM37 fusion protein Ag3690
• Full Name: tripartite motif-containing 37
• Calculated Molecular Weight: 964 aa, 108 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC036012
• Gene Symbol: TRIM37
• Gene ID (NCBI): 4591
• RRID: AB_10640800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM37 (tripartite motif containing 37) is a member of the tripartite motif (RING, B-Box, and coiled-coil domains) family . Its gene mutations result in mulibrey nanism, which is a rare, autosomal-recessive growth disorder of prenatal onset, with characteristic dysmorphic features, pericardial constriction, and hepatomegaly .TRIM37 is located on chromosome 17q23, encoding a protein with an estimated molecular weight of 130 kDa or 89kDa. (PMID: 28098873, PMID: 29940807, PMID: 14757854)

Publications

Species	Application	Title
human	IHC	Transl Oncol; TRIM37 promotes gallbladder cancer proliferation by activating the Wnt/β-catenin pathway via ubiquitination of Axin1; Authors - Ming Xu
human	WB,IHC,IP	Exp Cell Res; Aerobic glycolysis and tumor progression of hepatocellular carcinoma are mediated by ubiquitin of P53 K48-linked regulated by TRIM37; Authors - Yuzhen Ge ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Tripartite motif containing protein 37 involves in thrombin stimulated BV-2 microglial cell apoptosis and interleukin 1β release.; Authors - Chong Han ; KD Validated
human	WB,IHC	Cell Death Discov; KIFC1 depends on TRIM37-mediated ubiquitination of PLK4 to promote centrosome amplification in endometrial cancer; Authors - Kening Zhou
human	WB	Mol Cancer Res; Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis; Authors - Xinyi Liao
mouse	WB,IF	Mol Med; TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation; Authors - Hang Yang ; KD Validated