TRIM35 Polyclonal antibody

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  WB analysis of A431 using 19806-1-AP

  WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells.

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  WB analysis using 19806-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 19806-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TRIM35 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 19806-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

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Tested Applications

• Positive WB detected in: H9C2 cells, A431 cells, HEK-293 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19806-1-AP targets TRIM35 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM35 fusion protein Ag13584
• Full Name: tripartite motif-containing 35
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 45~50 kDa
• GenBank Accession Number: BC018337
• Gene Symbol: TRIM35
• Gene ID (NCBI): 23087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPQ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.