TRIM34 Recombinant antibody

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  WB analysis of K-562 using 83316-2-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 83316-2-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 83316-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83316-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

TRIM34 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83316-2-RR

Clone No.240180E2

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34

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Tested Applications

• Positive WB detected in: K-562 cells, human placenta tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83316-2-RR targets TRIM34 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM34 fusion protein Ag34444
• Full Name: tripartite motif-containing 34
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 31 kDa, 57 kDa
• GenBank Accession Number: NM_001003827
• Gene Symbol: TRIM34
• Gene ID (NCBI): 53840
• RRID: AB_3670982
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BYJ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).