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KAP1 Polyclonal antibody
KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 15202-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse testis tissue, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 9 publications below |
WB | See 32 publications below |
IHC | See 7 publications below |
IF | See 4 publications below |
IP | See 5 publications below |
CoIP | See 1 publications below |
ChIP | See 3 publications below |
Product Information
15202-1-AP targets KAP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KAP1 fusion protein Ag7350 |
Full Name | tripartite motif-containing 28 |
Calculated Molecular Weight | 89 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC004978 |
Gene Symbol | KAP1 |
Gene ID (NCBI) | 10155 |
RRID | AB_2209890 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )
Protocols
Product Specific Protocols | |
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WB protocol for KAP1 antibody 15202-1-AP | Download protocol |
IHC protocol for KAP1 antibody 15202-1-AP | Download protocol |
IF protocol for KAP1 antibody 15202-1-AP | Download protocol |
IP protocol for KAP1 antibody 15202-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers | ||
Hepatology Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.
| ||
J Exp Med ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons. | ||
Nucleic Acids Res Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.
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Proc Natl Acad Sci U S A TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (06-12-2024) | Very strong signal detected for TRIM28 (KAP1) by WB with HeLa and Jurkat extracts with an 1/1000 dilution solution and 1h incubation at RT.
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FH Katherine (Verified Customer) (09-25-2023) | Nice quality!
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FH Henar (Verified Customer) (09-03-2022) | Great antibody very specific, I will use always
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FH Isabelle Cristine (Verified Customer) (01-14-2022) | This antibody works extremely well and is very specific for detecting the KAP1 protein in U2OS cells.
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