• Featured Product
  • KD/KO Validated

TRIM27 Polyclonal antibody

TRIM27 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12205-1-AP

Synonyms

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP



Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue, Raji cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12205-1-AP targets TRIM27 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM27 fusion protein Ag2849
Full Name tripartite motif-containing 27
Calculated Molecular Weight 513 aa, 58 kDa
Observed Molecular Weight 58 kDa, 41 kDa
GenBank Accession NumberBC013580
Gene Symbol TRIM27
Gene ID (NCBI) 5987
RRIDAB_2256660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM27 antibody 12205-1-APDownload protocol
IHC protocol for TRIM27 antibody 12205-1-APDownload protocol
IP protocol for TRIM27 antibody 12205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Elife

The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.

Authors - Zane A Gibbs
humanCoIP

Mol Ther Nucleic Acids

TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination.

Authors - Li Xing
humanWB

FASEB J

TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.

Authors - Yinfang Wang
  • KD Validated
humanWB,IF

J Cell Mol Med

NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.

Authors - Bowen Hu
mouseWB

J Leukoc Biol

Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes.

Authors - Tongjie Wang
humanWB,IF

FEBS J

TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1

Authors - Juncal Garcia‐garcia
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Amy (Verified Customer) (03-18-2024)

Clean bands for U2OS cell lysates 25ug.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
FH

Jason (Verified Customer) (08-17-2022)

Third time purchase. I am satisfied. This antibody works very well in human breast cancer cells using WB at dilution 1:1000, but many non-specific IF singling detected.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WB: 1:1000
FH

Elisa (Verified Customer) (12-21-2021)

HAP1 were lysates with RIPA buffer. 1mg of protein was loaded per condition. Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with Trim27 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect TRIM27 signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HAP1
TRIM27 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:12205-1-AP)
FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (30 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-TRIM27 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T
TRIM27 Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:12205-1-AP)
FH

Francesca (Verified Customer) (05-16-2019)

Excellent antibody, clean and strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS and A549 cells
TRIM27 Antibody Western Blot, validation (1:2000 dilution) in U2OS and A549 cells (Cat no:12205-1-AP)