TRIM26 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM26 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 27013-1-AP

Synonyms

Acid finger protein, AFP, RING finger protein 95, RNF95, TRIM26, tripartite motif containing 26, Zinc finger protein 173, ZNF173

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Validation Data Gallery

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  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 27013-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27013-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27013-1-AP (TRIM26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 27013-1-AP

  IP result of anti-TRIM26 (IP:27013-1-AP, 4ug; Detection:27013-1-AP 1:300) with HeLa cells lysate 2400ug.

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27013-1-AP (TRIM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse testis tissue, RAW 264.7 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

27013-1-AP targets TRIM26 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM26 fusion protein Ag25546
• Full Name: tripartite motif-containing 26
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC024039
• Gene Symbol: TRIM26
• Gene ID (NCBI): 7726
• RRID: AB_2880721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma; Authors - Zhangjie Wang
pig	WB	Virus Res; TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection.; Authors - Pandeng Zhao
mouse	WB	Cell Death Dis; USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression; Authors - Weiwei Wang
human	WB,IHC,IF,IP	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun ; KD Validated
human	WB,IF	FASEB J; TRIM26 restricts Epstein-Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β; Authors - Mingjiao Zhang ; KD Validated
human	WB	Aging (Albany NY); Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation; Authors - Xiaocheng Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (03-20-2024)

Protein quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: HEK293T

TRIM26 Antibody Western Blot validation (1:2500 dilution) in HEK293T (Cat no:27013-1-AP)