TRIM25 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TRIM25 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H6G1

Cat no : 67314-1-PBS

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

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Validation Data Gallery

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  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67314-1-PBS targets TRIM25 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM25 fusion protein Ag29325
• Full Name: tripartite motif-containing 25
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 68-71 kDa
• GenBank Accession Number: BC016924
• Gene Symbol: TRIM25
• Gene ID (NCBI): 7706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).