TRIM24 Monoclonal antibody, PBS Only

TRIM24 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6D9

Cat no : 66324-1-PBS

Synonyms

hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1 alpha, TIF1A, TIF1ALPHA, TRIM24, tripartite motif containing 24

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Validation Data Gallery

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  WB analysis using 66324-1-Ig (same clone as 66324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66324-1-Ig (same clone as 66324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66324-1-Ig (same clone as 66324-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66324-1-Ig (TRIM24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66324-1-Ig (same clone as 66324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66324-1-Ig (TRIM24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66324-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66324-1-PBS targets TRIM24 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM24 fusion protein Ag5417
• Full Name: tripartite motif-containing 24
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC028689
• Gene Symbol: TRIM24
• Gene ID (NCBI): 8805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) which is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.