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  • KD/KO Validated

TRIM2 Polyclonal antibody

TRIM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20356-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 300 μg/ml
20 μL, 300 μg/ml
150 μL, 300 μg/ml

$449/ 150 μL

In stock in Rosemont, IL. Order within 3 hours and 33 mins to receive by Wednesday, April 2

Freight/Packing: $40.00

Quantity

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.


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Tested Applications

Positive WB detected inmouse brain tissue, mouse small intestine tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20356-1-AP targets TRIM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM2 fusion protein Ag14208 Predict reactive species
Full Name tripartite motif-containing 2
Calculated Molecular Weight 744 aa, 82 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC011052
Gene Symbol TRIM2
Gene ID (NCBI) 23321
RRIDAB_10734319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Protocols

Product Specific Protocols
WB protocol for TRIM2 antibody 20356-1-APDownload protocol
IHC protocol for TRIM2 antibody 20356-1-APDownload protocol
IF protocol for TRIM2 antibody 20356-1-APDownload protocol
IP protocol for TRIM2 antibody 20356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB,IF

Nat Commun

Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity

Authors - Diego Esposito
humanWB,IF

J Transl Med

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Authors - Zili Zhou
humanWB

Cancer Cell Int

TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.

Authors - Zhaoxian Lin
  • KD Validated
humanWB,IP,IF

FEBS J

TRIM2 promotes metabolic adaptation to glutamine deprivation via enhancement of CPT1A activity

Authors - Kaimin Liao
humanWB,IHC

Cell Mol Life Sci

E3 ubiquitin ligase TRIM2 identified as a novel suppressor of CYP11B2 and aldosterone production

Authors - Liang Chen
  • KD Validated
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