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TRIM15 Recombinant antibody

TRIM15 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240565C7

Cat no : 83582-5-RR

Synonyms

RNF93, TRIM, RING finger protein 93, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM15

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIM15 Recombinant antibody (83582-5-RR)

    IF Staining of HeLa using 83582-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM15 antibody (83582-5-RR, Clone: 240565C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83582-5-RR

    Affinity and Kinetic Characterization of 83582-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83582-5-RR against Human TRIM15 were performed. The affinity constant is 0.686 nM.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83582-5-RR is TRIM15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM15 fusion protein Ag4533
Full Name tripartite motif-containing 15
Calculated Molecular Weight 465 aa, 52 kDa
GenBank Accession NumberBC038585
Gene Symbol TRIM15
Gene ID (NCBI) 89870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM15 (Tripartite motif-containing protein 15) is also named as RNF93, ZNF178, ZNFB7 and belongs to the TRIM/RBCC family. It acts as an ubiquitin-protein ligase and contributes to the endogenous restriction of retroviruses in cells.

Protocols

Product Specific Protocols
IF protocol for TRIM15 antibody 83582-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols