TRIM14 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15742-1-AP

Synonyms

Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129

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Validation Data Gallery

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  WB analysis using 15742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15742-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15742-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15742-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM14 antibody (15742-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, HL-60 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15742-1-AP targets TRIM14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM14 fusion protein Ag8158
• Full Name: tripartite motif-containing 14
• Calculated Molecular Weight: 442 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC006333
• Gene Symbol: TRIM14
• Gene ID (NCBI): 9830
• RRID: AB_2209324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu ; KO Validated
human	WB	Sci Rep; TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.; Authors - Josephine Hai ; KD Validated
human	WB	Sci Rep; TRIM14 regulates cell proliferation and invasion in osteosarcoma via promotion of the AKT signaling pathway.; Authors - Guoxing Xu ; KD Validated
human	WB	Sci Rep; Expression profiling of TRIM protein family in THP1-derived macrophages following TLR stimulation.; Authors - Mei-Xiu Jiang
human	WB,IHC,IF	Carcinogenesis; TRIM14 suppressed the progression of NSCLC via hexosamine biosynthesis pathway; Authors - Sisi Wei
human	WB	Front Pharmacol; Hypoxia-Induced Upregulation of lncRNA ELFN1-AS1 Promotes Colon Cancer Growth and Metastasis Through Targeting TRIM14 via Sponging miR-191-5p.; Authors - Xu Jing