TRIM11 Recombinant antibody

TRIM11 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242107F1

Cat no : 84651-3-RR

Synonyms

RING finger protein 92, Protein BIA1, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM11, BIA1

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Validation Data Gallery

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  WB analysis using 84651-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84651-3-RR (TRIM11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84651-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84651-3-RR against Human TRIM11 were performed. The affinity constant is 0.25 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84651-3-RR targets TRIM11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM11 fusion protein Ag1308
• Full Name: tripartite motif-containing 11
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011629
• Gene Symbol: TRIM11
• Gene ID (NCBI): 81559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.