TRH Polyclonal antibody

TRH Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25332-1-AP

Synonyms

Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor



Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25332-1-AP targets TRH in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRH fusion protein Ag12623
Full Name thyrotropin-releasing hormone
Calculated Molecular Weight 242 aa, 27 kDa
Observed Molecular Weight27-33 kDa
GenBank Accession NumberBC074889
Gene Symbol TRH
Gene ID (NCBI) 7200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).

Protocols

Product Specific Protocols
WB protocol for TRH antibody 25332-1-APDownload protocol
IF protocol for TRH antibody 25332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols