TRBP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TRBP Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A10

Cat no : 66792-1-PBS

Synonyms

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

---

Validation Data Gallery

• 

  WB analysis using 66792-1-Ig (same clone as 66792-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66792-1-Ig (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

---

Product Information

66792-1-PBS targets TRBP in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRBP fusion protein Ag8269
• Full Name: TAR (HIV-1) RNA binding protein 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC005860
• Gene Symbol: TRBP
• Gene ID (NCBI): 6895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)