TRAPPC9, NIBP Monoclonal antibody

TRAPPC9, NIBP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C4F9

Cat no : 66131-1-Ig

Synonyms

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRAPPC9,NIBP, TRS120, Tularik gene 1 protein

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Validation Data Gallery

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  WB analysis of human brain using 66131-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66131-1-Ig (TRAPPC9,NIBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66131-1-Ig (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66131-1-Ig (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66131-1-Ig is TRAPPC9, NIBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAPPC9, NIBP fusion protein Ag8949
• Full Name: trafficking protein particle complex 9
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 128 kDa
• GenBank Accession Number: BC006206
• Gene Symbol: NIBP/Trappc9
• Gene ID (NCBI): 83696
• RRID: AB_2881530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation and play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter.