TRAPPC9, NIBP Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAPPC9, NIBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 16014-1-AP

Synonyms

NIBP, TRAPPC9, TRAPPC9,NIBP, TRAPPC9/NIBP, NIK- and IKBKB-binding protein

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Validation Data Gallery

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  WB analysis of mouse brain using 16014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16014-1-AP

  IP result of anti-TRAPPC9, NIBP (IP:16014-1-AP, 4ug; Detection:16014-1-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human kidney using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 16014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16014-1-AP (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 16014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRAPPC9, NIBP antibody (16014-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16014-1-AP targets TRAPPC9, NIBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC9, NIBP fusion protein Ag8791
• Full Name: trafficking protein particle complex 9
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 128-140 kDa
• GenBank Accession Number: BC006206
• Gene Symbol: NIBP/Trappc9
• Gene ID (NCBI): 83696
• RRID: AB_2256482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC9, also named KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cell differentiation and play a role in vesicular transport from the endoplasmic reticulum to the Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. Western blotting indicates the molecular weight of TRAPPC9 is 130-140 kDa, and 250 kDa may be detected with extensive posttranslational modification of TRAPPC9.

Publications

Species	Application	Title
human	IF	EMBO J; The TRAPP complex mediates secretion arrest induced by stress granule assembly.; Authors - Francesca Zappa
human	IF	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
mouse,zebrafish	WB,IF	Int J Biol Sci; Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice; Authors - Min Hu
mouse	WB	EMBO Rep; TRAPPC1 is essential for the maintenance and differentiation of common myeloid progenitors in mice; Authors - Yanan Xu
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca ; KD Validated
human	WB,IHC	Tumour Biol; NIK- and IKKβ-binding protein promotes colon cancer metastasis by activating the classical NF-κB pathway and MMPs.; Authors - Mengbin Qin

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