TRAPPC4 Recombinant antibody

TRAPPC4 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241158C11

Cat no : 84119-3-RR

Synonyms

SBDN, PTD009, HSPC172, CGI-104, CGI 104

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Validation Data Gallery

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  WB analysis using 84119-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 84119-3-RR

  IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

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  Affinity and Kinetic Characterization of 84119-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HCT 116 cells, HL-60 cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84119-3-RR is TRAPPC4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRAPPC4 fusion protein Ag2737
• Full Name: trafficking protein particle complex 4
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC010866
• Gene Symbol: TRAPPC4
• Gene ID (NCBI): 51399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).