Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12074-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP experiment of HL-60 using 12074-1-AP
IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.
IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.
IHC staining of human colon cancer using 12074-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12074-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12074-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
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HL-60 cells, mouse testis tissue, rat testis tissue
Positive IP detected in
HL-60 cells
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
12074-1-AP targets TRAPPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).
Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12074-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IHC Figures
IHC staining of human colon cancer using 12074-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12074-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HL-60 using 12074-1-AP
IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.
IF/ICC Figures
IF Staining of HeLa using 12074-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TRAPPC4 Polyclonal antibody
Catalog Number
12074-1-AP
Citations
-
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
