TRAPA/SSR1 Monoclonal antibody, PBS Only

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2D7

Cat no : 67925-1-PBS

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

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Validation Data Gallery

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  WB analysis using 67925-1-Ig (same clone as 67925-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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Product Information

67925-1-PBS targets TRAPA/SSR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAPA/SSR1 fusion protein Ag30832
• Full Name: signal sequence receptor, alpha
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007710
• Gene Symbol: TRAPA
• Gene ID (NCBI): 6745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).