TRAK1 Polyclonal antibody

TRAK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13987-1-AP

Synonyms

KIAA1042, OIP106, TRAK1

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Validation Data Gallery

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  WB analysis using 13987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13987-1-AP (TRAK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13987-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human stomach using 13987-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human breast cancer using 13987-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13987-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MNK-45 cells
• Positive IHC detected in: human stomach tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:800
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13987-1-AP targets TRAK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAK1 fusion protein Ag5055
• Full Name: trafficking protein, kinesin binding 1
• Calculated Molecular Weight: 953 aa, 106 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC015922
• Gene Symbol: TRAK1
• Gene ID (NCBI): 22906
• RRID: AB_2877999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Neurosci; O-GlcNAcylation is crucial for sympathetic neuron development, maintenance, functionality and contributes to peripheral neuropathy; Authors - Hsueh-Fu Wu