TRAIL Recombinant antibody, PBS Only (Capture)

TRAIL Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241419A2

Cat no : 84280-2-PBS

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP01166-1

  Sandwich ELISA standard curve of MP01166-1, Human TRAIL Recombinant Matched Antibody Pair - PBS only. 84280-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02339. 84280-1-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL

• 

  Sample test of MP01166-1

  Serum of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 182.8 pg/mL with a range of 33.7 - 368.3 pg/mL.

• 

  Sample test of MP01166-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TRAIL concentration was undetectable in unstimulated PBMC supernatant, 22.3 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TRAIL concentration was determined to be 1.0 pg/mL in unstimulated PBMC supernatant, 22.9 pg/mL in PHA stimulated PBMC supernatant.

• 

  Sample test of MP01166-1

  Saliva of six individual healthy human donors was measured. The human TRAIL concentration of detected samples was determined to be 809.9 pg/mL with a range of 159.6 - 1,340.0 pg/mL.

• 

  Cytometric bead array standard curve of MP01166-1

  Cytometric bead array standard curve of MP01166-1, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-1-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

• 

  Cytometric bead array standard curve of MP01166-2

  Cytometric bead array standard curve of MP01166-2, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-3-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

• 

  Cytometric bead array standard curve of MP01166-3

  Cytometric bead array standard curve of MP01166-3, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-4-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

---

Product Information

84280-2-PBS targets TRAIL as part of a matched antibody pair:

MP01166-1: 84280-2-PBS capture and 84280-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01166-2: 84280-2-PBS capture and 84280-3-PBS detection (validated in Cytometric bead array)

MP01166-3: 84280-2-PBS capture and 84280-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.