TRAF6 Monoclonal antibody, PBS Only

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  WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66498-1-Ig (same clone as 66498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66498-1-Ig (same clone as 66498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66498-1-Ig (same clone as 66498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66498-1-Ig (same clone as 66498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66498-1-Ig (same clone as 66498-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF6 antibody (66498-1-Ig, Clone: 1D1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRAF6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66498-1-PBS

Clone No.1D1E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNF85, RING-type E3 ubiquitin transferase TRAF6, Interleukin-1 signal transducer, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6

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Product Information

66498-1-PBS targets TRAF6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF6 fusion protein Ag24696
• Full Name: TNF receptor-associated factor 6
• Calculated Molecular Weight: 522 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC031052
• Gene Symbol: TRAF6
• Gene ID (NCBI): 7189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4K3
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.