• Featured Product
  • KD/KO Validated

TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1D1E1

Cat no : 66498-1-Ig

Synonyms

RING finger protein 85, RNF85, TRAF6



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HepG2 cells, U2OS cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66498-1-Ig targets TRAF6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, pig, fish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF6 fusion protein Ag24696
Full Name TNF receptor-associated factor 6
Calculated Molecular Weight 522 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
RRIDAB_2881862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Protocols

Product Specific Protocols
WB protocol for TRAF6 antibody 66498-1-IgDownload protocol
IHC protocol for TRAF6 antibody 66498-1-IgDownload protocol
IF protocol for TRAF6 antibody 66498-1-IgDownload protocol
FC protocol for TRAF6 antibody 66498-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Autophagy

Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis

Authors - Jiajun Liu
humanWB

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
humanWB

Cell Death Differ

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Authors - Shunfeng Hu
humanIHC

J Immunother Cancer

Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.

Authors - Yuwei Deng
humanWB

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
WB

Theranostics

Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.

Authors - Junjian Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Andrea (Verified Customer) (12-07-2018)

Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.

  • Applications: Western Blot, Immunoprecipitation,