TRAF5 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66991-1-Ig (same clone as 66991-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66991-1-Ig (same clone as 66991-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 66991-1-Ig (same clone as 66991-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

TRAF5 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66991-1-PBS

Clone No.1B1A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

RING finger protein 84, RNF84, TRAF5

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Product Information

66991-1-PBS targets TRAF5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF5 fusion protein Ag26070
• Full Name: TNF receptor-associated factor 5
• Calculated Molecular Weight: 557 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC029600
• Gene Symbol: TRAF5
• Gene ID (NCBI): 7188
• RRID: AB_2882308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00463
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299). The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).