TRAF5 Polyclonal antibody

TRAF5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26917-1-AP

Synonyms

RING finger protein 84, RNF84, TRAF5

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Validation Data Gallery

• 

  WB analysis of Jurkat using 26917-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26917-1-AP (TRAF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26917-1-AP is TRAF5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF5 fusion protein Ag25284
• Full Name: TNF receptor-associated factor 5
• Calculated Molecular Weight: 557 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC029600
• Gene Symbol: TRAF5
• Gene ID (NCBI): 7188
• RRID: AB_2880684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299).

The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).

What is the molecular weight of TRAF5?

TRAF5 is a 557 amino acid protein that has a predicted molecular weight of 64 kDa.

What is the subcellular localization of TRAF-5?

TRAF5 is a cytoplasmic protein (PMID:11607847).

What is the tissue specificity of TRAF5?

TRAF5 is broadly expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood. The highest level of expression is observed in Peyer's patches in the small intestine (https://www.uniprot.org/uniprot/O00463).

What is the role of TRAF5 and immune cell function?

Studies in traf5-/- mice have shown that TRAF5 plays a key role in CD40-mediated signaling in B-cells and CD27-mediated signaling in T-cells. In B-cells, deletion of the traf5 gene results in defects in cell proliferation and upregulation of various cell surface molecules, including CD23, CD54, CD80, CD86, and Fas, in response to CD40 stimulation. Similarly, upregulation of CD27-mediated costimulatory signals is also impaired in traf5−/− T cells. These effects lead to impairments in lymphocyte activation in vivo (PMID:10449775).

TRAF5 also plays a critical role in in vitro signaling and in vivo function in B-cells by the Epstein-Barr Virus (EBV)-encoded oncogenic mimic of CD40, latent membrane protein 1 (LMP1), which is encoded by the virus and is essential for transformation of B lymphocytes (PMID:19805155).