TRAF4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9A11

Cat no : 66755-1-PBS

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

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Validation Data Gallery

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  WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66755-1-Ig (same clone as 66755-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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Product Information

66755-1-PBS targets TRAF4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF4 fusion protein Ag27221
• Full Name: TNF receptor-associated factor 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC001769
• Gene Symbol: TRAF4
• Gene ID (NCBI): 9618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.