TRAF1 Polyclonal antibody

TRAF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11543-1-AP

Synonyms

EBI6, TRAF1

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Validation Data Gallery

• 

  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11543-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11543-1-AP (TRAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11543-1-AP targets TRAF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF1 fusion protein Ag2131
• Full Name: TNF receptor-associated factor 1
• Calculated Molecular Weight: 416 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC024145
• Gene Symbol: TRAF1
• Gene ID (NCBI): 7185
• RRID: AB_2287879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF1 is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. TRAF1 and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. TRAF1 is observed at 45-50 kDa in western blotting, and the additional bands of lower molecular weight (~30 kDa) may represent TRAF1 cleavage fragments, similar to the results obtained by the article (PMID:11098060).

Publications

Species	Application	Title
mouse	WB	Eur J Nutr; Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.; Authors - Mengmeng You