TRA2B Polyclonal antibody

• Featured Product
• KD/KO Validated

TRA2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29627-1-AP

Synonyms

TRA 2 beta, SRFS10, Splicing factor, arginine/serine-rich 10, SFRS10, hTRA2-beta

---

Validation Data Gallery

• 

  WB analysis using 29627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29627-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 29627-1-AP (TRA2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 29627-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29627-1-AP (TRA2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

29627-1-AP targets TRA2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRA2B fusion protein Ag30637
• Full Name: transformer 2 beta homolog (Drosophila)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC005898
• Gene Symbol: TRA2B
• Gene ID (NCBI): 6434
• RRID: AB_2918335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transformer-2 protein homolog beta, also known as TRA2B, is a sequence-specific RNA-binding protein that participates in the control of pre-mRNA splicing. TRA2B can alter pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX.

Publications

Species	Application	Title
human	WB,IHC	Biomed Environ Sci; Tra2β Enhances Cell Proliferation by Inducing the Expression of Transcription Factor SP1 in Cervical Cancer; Authors - Mo Juan Li ; KD Validated