TPSAB1 Polyclonal antibody, PBS Only

TPSAB1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13343-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13343-1-AP (same clone as 13343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 13343-1-AP (same clone as 13343-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 13343-1-AP (same clone as 13343-1-PBS)

  IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendix using 13343-1-AP (same clone as 13343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 13343-1-AP (same clone as 13343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13343-1-AP (same clone as 13343-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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  FC experiment of U-937 using 13343-1-AP (same clone as 13343-1-PBS)

  1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 13343-1-PBS in a different storage buffer formulation.

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Product Information

13343-1-PBS targets TPSAB1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPSAB1 fusion protein Ag4167
• Full Name: tryptase alpha/beta 1
• Calculated Molecular Weight: 275 aa, 31 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: BC028059
• Gene Symbol: TPSAB1
• Gene ID (NCBI): 7177
• RRID: AB_2206197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.