TPRG1L Recombinant antibody

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  WB analysis using 83989-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83989-4-RR (TPRG1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83989-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83989-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83989-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83989-4-RR against Human TPRG1L were performed. The affinity constant is 0.234 nM.

TPRG1L Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83989-4-RR

Clone No.241070G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

241070G6, FAM79A, h mover, Mossy fiber terminal-associated vertebrate-specific presynaptic protein, MOVER

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83989-4-RR targets TPRG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TPRG1L fusion protein Ag22585
• Full Name: tumor protein p63 regulated 1-like
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC019034
• Gene Symbol: TPRG1L
• Gene ID (NCBI): 127262
• RRID: AB_3671565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5T0D9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPRG1L (Tumor protein p63-regulated gene 1-like protein) belongs to the presynaptic protein involved in the synaptic transmission tuning. It can regulate synaptic release probability by decreasing the calcium sensitivity of release. TPRG1L has two isoforms with molecular weights of 30kd and 23kd respectively. This antibody can recognize the 30kd isoform and may also recognize the 23kd isoform.