TPRG1L Recombinant antibody, PBS Only (Detector)

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  WB analysis using 83989-3-RR (same clone as 83989-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83989-3-RR (TPRG1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83989-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83989-3-RR (same clone as 83989-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-3-RR (TPRG1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83989-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83989-3-RR (same clone as 83989-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-3-RR (TPRG1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83989-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00883-1

  Cytometric bead array standard curve of MP00883-1, TPRG1L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83989-1-PBS. Detection antibody: 83989-3-PBS. Standard: Ag22585. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00883-3

  Cytometric bead array standard curve of MP00883-3, TPRG1L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83989-2-PBS. Detection antibody: 83989-3-PBS. Standard: Ag22585. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83989-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83989-3-RR against Human TPRG1L were performed. The affinity constant is 0.954 nM.

TPRG1L Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 83989-3-PBS

Clone No.241070B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

241070B8, FAM79A, h mover, Mossy fiber terminal-associated vertebrate-specific presynaptic protein, MOVER

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Product Information

83989-3-PBS targets TPRG1L as part of a matched antibody pair:

MP00883-1: 83989-1-PBS capture and 83989-3-PBS detection (validated in Cytometric bead array)

MP00883-3: 83989-2-PBS capture and 83989-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TPRG1L fusion protein Ag22585
• Full Name: tumor protein p63 regulated 1-like
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019034
• Gene Symbol: TPRG1L
• Gene ID (NCBI): 127262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T0D9
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Presynaptic protein involved in the synaptic transmission tuning. Regulates synaptic release probability by decreasing the calcium sensitivity of release.