TPPP Polyclonal antibody

TPPP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18742-1-AP

Synonyms

25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1

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Validation Data Gallery

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  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18742-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18742-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, mouse spleen tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18742-1-AP targets TPPP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulin polymerization promoting protein
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC131506
• Gene Symbol: TPPP
• Gene ID (NCBI): 11076
• RRID: AB_2256275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Publications

Species	Application	Title
mouse	IF	Cell; The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination.; Authors - Meng-Meng Fu
human	WB,IHC,IF	FASEB J; EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.; Authors - Yingfen Wu
human	WB	Stem Cells Int; Enhanced Effect of IL-1β-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling.; Authors - Liu Liu
	WB	ACS Infect Dis; Repositioning Brusatol as a Transmission Blocker of Malaria Parasites; Authors - Amelia Cox
rat	WB	J Mol Cell Cardiol; Defective autophagy triggered by arterial cyclic stretch promotes neointimal hyperplasia in vein grafts via the p62/nrf2/slc7a11 signaling pathway; Authors - Yi Chen