TPM3 Monoclonal antibody

TPM3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10B3

Cat no : 68158-1-Ig

Synonyms

Tropomyosin-5, Tropomyosin-3, Tropomyosin alpha-3 chain, Tropomyosin alpha 3 chain, Gamma-tropomyosin

---

Validation Data Gallery

• 

  WB analysis using 68158-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 68158-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68158-1-Ig (TPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of C2C12 using 68158-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TPM3 antibody (68158-1-Ig, Clone: 1F10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Tested Applications

• Positive WB detected in: pig heart tissue, U2OS cells, Rabbit heart tissue, Rat heart tissue, Mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68158-1-Ig targets TPM3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPM3 fusion protein Ag28568
• Full Name: tropomyosin 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-37 kDa
• GenBank Accession Number: BC008407
• Gene Symbol: TPM3
• Gene ID (NCBI): 7170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.