TPM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TPM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55443-1-AP

Synonyms

AMCD1, Beta tropomyosin, DA1, DA2B, TMSB, TPM2, Tropomyosin 2, tropomyosin 2 (beta), Tropomyosin beta chain

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Validation Data Gallery

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  WB analysis of mouse heart using 55443-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55443-1-AP (TPM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55443-1-AP

  WB result of TPM2 antibody (55443-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM2 transfected HeLa cells.

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  IHC staining of human breast cancer using 55443-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55443-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55443-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55443-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55443-1-AP targets TPM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tropomyosin 2 (beta)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_003289
• Gene Symbol: TPM2
• Gene ID (NCBI): 7169
• RRID: AB_2881335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.