TPM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TPM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11038-1-AP

Synonyms

Tropomyosin 2, TMSB, DA1, Beta-tropomyosin, Beta tropomyosin

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Validation Data Gallery

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  WB analysis using 11038-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11038-1-AP (TPM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11038-1-AP

  WB result of TPM2 antibody (11038-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM2 transfected HeLa cells.

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  IP experiment of mouse skeletal muscle using 11038-1-AP

  IP result of anti-TPM2 (IP:11038-1-AP, 4ug; Detection:11038-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human breast cancer using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11038-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11038-1-AP (TPM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 11038-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11038-1-AP (TPM2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11038-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPM2 antibody (11038-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidinv (red).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, mouse skeletal muscle, rat skeletal muscle
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human breast cancer tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

11038-1-AP targets TPM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPM2 fusion protein Ag1508
• Full Name: tropomyosin 2 (beta)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 29-33 kDa
• GenBank Accession Number: BC011776
• Gene Symbol: TPM2
• Gene ID (NCBI): 7169
• RRID: AB_2205660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPM2, also named as TMSB, belongs to the tropomyosin family. It binds to actin filaments in muscle and non-muscle cells. TPM2 plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. In non-piamuscle cells, TPM2 is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4) and distal arthrogryposis type 1 (DA1).

Publications

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human,mouse	WB,IHC,IP	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono
rat	WB	Int J Mol Sci; Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle; Authors - Beatrice Vahle

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-03-2024)

A view unspecific bands near the region of the protein, but enough blocking (o.N.) got rid of the problem. A well visible band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs