TP63 Monoclonal antibody, PBS Only

TP63 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D6G12

Cat no : 60332-2-PBS

Synonyms

p63, CUSP, tumor protein p63, Tumor protein 63, Transformation-related protein 63

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Validation Data Gallery

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  WB analysis of HaCaT using 60332-2-Ig (same clone as 60332-2-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60332-2-Ig (same clone as 60332-2-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60332-2-Ig (same clone as 60332-2-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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Product Information

60332-2-PBS targets TP63 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TP63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated Molecular Weight: 680 aa, 77 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039815
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.