TOX4 Polyclonal antibody

TOX4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27988-1-AP

Synonyms

TOX 4, MIG7, LCP1, C14orf92

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Validation Data Gallery

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  WB analysis using 27988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27988-1-AP (TOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 27988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 27988-1-AP (TOX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27988-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOX4 antibody (27988-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, MCF-7 cells, PC-3 cells, Raji cells
• Positive IHC detected in: rat bladder tissue, mouse bladder tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27988-1-AP targets TOX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOX4 fusion protein Ag27638
• Full Name: TOX high mobility group box family member 4
• Calculated Molecular Weight: 621 aa, 66 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC013689
• Gene Symbol: TOX4
• Gene ID (NCBI): 9878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOX (Thymocyte selection-associated HMG bOX) genes represent a novel gene family and encode a novel nuclear DNA binding protein belonging to a large superfamily of HMG (high mobility group)-box family. TOX proteins consist a small subfamily of proteins, including TOX1, TOX2, TOX3, and TOX4. The TOX subfamily members are highly conserved among vertebrates. TOX4 was recently found to be one of the subunits of the PP1C, which consists of three regulatory proteins, PNUTS, TOX4, and WDR82, and one of the PP1 phosphatases, PP1α, β, andγ25. Tox4 can modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (PMID:31519808). Despite a predicted molecular mass of TOX4 protein of 66 kDa, the detection band of western blot analysis in many literatures is 100 kDa (PMID:37286708;35365735). This antibody was also detected at around 100 kda.