TOX2 Recombinant antibody, PBS Only (Capture)

TOX2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241645A11

Cat no : 84304-2-PBS

Synonyms

GCX-1, GCX1, C20orf100, 241645A11

---

Validation Data Gallery

• 

  IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 84304-2-RR (same clone as 84304-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 84304-2-RR (same clone as 84304-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01188-1

  Cytometric bead array standard curve of MP01188-1, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-1-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

• 

  Cytometric bead array standard curve of MP01188-2

  Cytometric bead array standard curve of MP01188-2, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-4-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

• 

  Cytometric bead array standard curve of MP01188-3

  Cytometric bead array standard curve of MP01188-3, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-3-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84304-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84304-2-RR against Human TOX2 were performed. The affinity constant is 0.240 nM.

---

Product Information

84304-2-PBS targets TOX2 as part of a matched antibody pair:

MP01188-1: 84304-2-PBS capture and 84304-1-PBS detection (validated in Cytometric bead array)

MP01188-2: 84304-2-PBS capture and 84304-4-PBS detection (validated in Cytometric bead array)

MP01188-3: 84304-2-PBS capture and 84304-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOX2 fusion protein Ag14772
• Full Name: TOX high mobility group box family member 2
• Calculated Molecular Weight: 488 aa, 52 kDa
• GenBank Accession Number: BC007636
• Gene Symbol: TOX2
• Gene ID (NCBI): 84969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).