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TOX2 Polyclonal antibody, PBS Only

TOX2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30474-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOX2 Polyclonal antibody (30474-1-AP)

    WB analysis using 30474-1-AP (same clone as 30474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30474-1-AP (TOX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30474-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TOX2 Polyclonal antibody (30474-1-AP)

    IF Staining of A431 using 30474-1-AP (same clone as 30474-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOX2 antibody (30474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30474-1-PBS in a different storage buffer formulation.

Product Information

30474-1-PBS targets TOX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOX2 fusion protein Ag31226
Full Name TOX high mobility group box family member 2
Calculated Molecular Weight 488 aa, 52 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC007636
Gene Symbol TOX2
Gene ID (NCBI) 84969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).