TOR3A Polyclonal antibody

• Featured Product
• KD/KO Validated

TOR3A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10647-1-AP

Synonyms

torsin family 3, member A, Torsin family 3 member A, Torsin 3A, ATP-dependent interferon-responsive protein, ADIR2

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Validation Data Gallery

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  WB analysis using 10647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10647-1-AP

  WB result of TOR3A antibody (10647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOR3A transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10647-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10647-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 10647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10647-1-AP (TOR3A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Raji cells, PC-3 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10647-1-AP targets TOR3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOR3A fusion protein Ag1012
• Full Name: torsin family 3, member A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 39-42 kDa, 46-52 kDa
• GenBank Accession Number: BC007571
• Gene Symbol: TOR3A
• Gene ID (NCBI): 64222
• RRID: AB_2303598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.