TOP2B Recombinant antibody

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  WB analysis using 84049-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84049-1-RR (TOP2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84049-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (84049-1-RR, Clone: 240949F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TOP2B Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84049-1-RR

Clone No.240949F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TOPIIB, top2beta, EC:5.6.2.2, DNA topoisomerase 2-beta, 240949F10

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, HEK-293T cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84049-1-RR targets TOP2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: topoisomerase (DNA) II beta 180kDa
• Calculated Molecular Weight: 183 kDa
• Observed Molecular Weight: 180-190 kDa
• GenBank Accession Number: NM_001068
• Gene Symbol: TOP2B
• Gene ID (NCBI): 7155
• RRID: AB_3671617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q02880
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.