TOM70 Monoclonal antibody, PBS Only

TOM70 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8A1

Cat no : 66593-1-PBS

Synonyms

KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

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Validation Data Gallery

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  WB analysis using 66593-1-Ig (same clone as 66593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66593-1-PBS targets TOM70 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TOM70 fusion protein Ag6027
• Full Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC052994
• Gene Symbol: TOMM70A
• Gene ID (NCBI): 9868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.