CoraLite®555-conjugated TOM20 Monoclonal antibody

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  IF Staining of HepG2 using CL555-66777

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 TOM20 antibody (CL555-66777, Clone: 1D6F5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66777

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human TOM20 (CL555-66777, Clone:1D6F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TOM20 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-66777

Clone No.1D6F5

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66777 targets TOM20 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TOM20 fusion protein Ag2378
• Full Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Calculated Molecular Weight: 145 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000882
• Gene Symbol: TOM20
• Gene ID (NCBI): 9804
• RRID: AB_2919694
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15388
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.